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An Electron Cloud Distribution Observed by the Scanning Seebeck Microscope
All matters are made of small particles, namely atoms. An atom is composed of a heavy nucleus and cloud-like, extremely light electrons.
Korean researchers developed an electron microscopy technique that enables the accurate observation of an electron cloud distribution at room-temperature. The achievement is comparable to the invention of the quantum tunneling microscopy technique developed 33 years ago.
Professor Yong-Hyun Kim of the Graduate School of Nanoscience and Technology at KAIST and Dr. Ho-Gi Yeo of the Korea Research Institute of Standards and Science (KRISS) developed the Scanning Seebeck Microscope (SSM). The SSM renders clear images of atoms, as well as an electron cloud distribution. This was achieved by creating a voltage difference via a temperature gradient.
The development was introduced in the online edition of Physical Review Letters (April 2014), a prestigious journal published by the American Institute of Physics.
The SSM is expected to be economically competitive as it gives high resolution images at an atomic scale even for graphene and semiconductors, both at room temperature. In addition, if the SSM is applied to thermoelectric material research, it will contribute to the development of high-efficiency thermoelectric materials.
Through numerous hypotheses and experiments, scientists now believe that there exists an electron cloud surrounding a nucleus. IBM's Scanning Tunneling Microscope (STM) was the first to observe the electron cloud and has remained as the only technique to this day. The developers of IBM microscope, Dr. Gerd Binnig and Dr. Heinrich Rohrer, were awarded the 1986 Nobel Prize in Physics.
There still remains a downside to the STM technique, however: it required high precision and extreme low temperature and vibration. The application of voltage also affects the electron cloud, resulting in a distorted image.
The KAIST research team adopted a different approach by using the Seebeck effect which refers to the voltage generation due to a temperature gradient between two materials.
The team placed an observation sample (graphene) at room temperature (37~57℃) and detected its voltage generation. This technique made it possible to observe an electron cloud at room temperature.
Furthermore, the research team investigated the theoretical quantum mechanics behind the electron cloud using the observation gained through the Seebeck effect and also obtained by simulation capability to analyze the experimental results.
The research was a joint research project between KAIST Professor Yong-Hyun Kim and KRISS researcher Dr. Ho-Gi Yeo. Eui-Seop Lee, a Ph.D. candidate of KAIST, and KRISS researcher Dr. Sang-Hui Cho also participated. The Ministry of Science, ICT, and Future Planning, the Global Frontier Initiative, and the Disruptive Convergent Technology Development Initiative funded the project in Korea.
Picture 1: Schematic Diagram of the Scanning Seebeck Microscope (SSM)
Picture 2: Electron cloud distribution observed by SSM at room temperature
Picture 3: Professor Yong-Hyun Kim
2014.04.04 View 12926 -
Professor Kyung-Wook Paik Receives the Best Presentation Award from 2014 Pan Pacific Symposium
The Surface Mount Technology Association (SMTA) hosted its 19th Annual Pan Pacific Microelectronics Symposium on February 11-13, 2014 in Hawaii.
The 2014 conference, promoting international technical exchange and extensive networking among microelectronics professionals from around the world, presented over 50 papers from 17 countries.
Professor Kyung-Wook Paik of Materials Science Engineering at KAIST received the Best Presentation Award for his paper titled, “Novel Nanofiber Anisotropic Films for Nine Pitch Assembly” at the conference.
SMTA is an international network of professionals in electronics assembly technologies, including Microsystems, emerging technologies, and related business operations.
2014.03.17 View 8357 -
The Korea Herald: Synthetic Biology Holds Key to Future
The Korea Herald
, a leading English newspaper in Korea, published an article on the production of gasoline by a metabolically engineered microorganism. For the article, please go to the link below:
The Korea Herald
March 14, 2014
"Synthetic biology holds key to future"
KAIST professor makes breakthrough in biorefining using E. coli’s metabolic process
http://www.koreaherald.com/view.php?ud=20140314001343
2014.03.17 View 6943 -
A Molecular Switch Controlling Self-Assembly of Protein Nanotubes Discovered
International collaborative research among South Korea, United States, and Israel research institutionsThe key to the treatment of cancer and brain disease mechanism The molecular switch that controls the self-assembly structure of the protein nanotubes, which plays crucial role in cell division and intracellular transport of materials, has been discovered. KAIST Bio and Brain Engineering Department’s Professor Myeong-Cheol Choi and Professor Chae-Yeon Song conducted the research, in collaboration with the University of California in Santa Barbara, U.S., and Hebrew University in Israel. The findings of the research were published in Nature Materials on the 19th. Microtubules are tube shaped and composed of protein that plays a key role in cell division, cytoskeleton, and intercellular material transport and is only 25nm in diameter (1/100,000 thickness of a human hair). Conventionally, cancer treatment focused on disrupting the formation of microtubules to suppress the division of cancer cells. In addition Alzheimer’s is known to be caused by the diminishing of structural integrity of microtubules responsible for intercellular material transport which leads to failure in signal transfer. The research team utilized synchrotron x-ray scattering and transmission electron microscope to analyze the self assemble structure of protein nanotubes to subnanometer accuracy. As a result, the microtubules were found to assemble into 25nm thickness tubules by stacking protein blocks 4 x 5 x 8nm in dimension. In the process, the research team discovered the molecular switch that controls the shape of these protein blocks. In addition the research team was successful in creating a new protein tube structure. Professor Choi commented that they were successful in introducing a new paradigm that suggests the possibility of controlling the complex biological functions of human’s biological system with the simple use of physical principles. He commented further that it is anticipated that the findings will allow for the application of bio nanotubes in engineering and that this is a small step in finding the mechanism behind cancer treatment and neural diseases.
2014.02.03 View 9775 -
KAIST Takes Steps towards a Self-Sustainable Campus
KAIST has been selected for a $45-million national smart grid initiative organized under the Ministry of Trade, Industry and Energy. Ninteen institutions will participate in the 2-year-long initiative. The consortium’s work is expected to take place from 2015 to 2017 after a review by the Ministry of Strategy and Finance.
The Smart Grid Explansion Initiative which has been considered the future of electric power industry implements information and communications technology to conventional grid system to maximize energy efficiency. The ROK government has selected the Smart Grid Expansion Initiative as one of South Korea’s primary national projects and plans to implement it nationwide based on multiple demonstration projects in major cities including Jeju.
KAIST plans to invest $45 million in developing systems for renewable energy power plants, efficient energy management, smart grid data, and electric vehicles to build the energy self-sustainable campus. It also hopes to contribute to fostering specialized talents and companies in energy management.
Byoung-Yoon Kim, the vice-president for research at KAIST, expects that by 2017, KAIST will be able to dramatically improve its energy capacity especially during peak periods and gain energy efficiency around the campus. He hopes that the micro grid project at KAIST will set a new standard for the self-sustainable campus.
2013.12.11 View 7797 -
KAIST announced a novel technology to produce gasoline by a metabolically engineered microorganism
A major scientific breakthrough in the development of renewable energy sources and other important chemicals; The research team succeeded in producing 580 mg of gasoline per liter of cultured broth by converting in vivo generated fatty acids
For many decades, we have been relying on fossil resources to produce liquid fuels such as gasoline, diesel, and many industrial and consumer chemicals for daily use. However, increasing strains on natural resources as well as environmental issues including global warming have triggered a strong interest in developing sustainable ways to obtain fuels and chemicals.
Gasoline, the petroleum-derived product that is most widely used as a fuel for transportation, is a mixture of hydrocarbons, additives, and blending agents. The hydrocarbons, called alkanes, consist only of carbon and hydrogen atoms. Gasoline has a combination of straight-chain and branched-chain alkanes (hydrocarbons) consisted of 4-12 carbon atoms linked by direct carbon-carbon bonds.
Previously, through metabolic engineering of Escherichia coli (E. coli), there have been a few research results on the production of long-chain alkanes, which consist of 13-17 carbon atoms, suitable for replacing diesel. However, there has been no report on the microbial production of short-chain alkanes, a possible substitute for gasoline.
In the paper (entitled "Microbial Production of Short-chain Alkanes") published online in Nature on September 29, a Korean research team led by Distinguished Professor Sang Yup Lee of the Department of Chemical and Biomolecular Engineering at the Korea Advanced Institute of Science and Technology (KAIST) reported, for the first time, the development of a novel strategy for microbial gasoline production through metabolic engineering of E. coli.
The research team engineered the fatty acid metabolism to provide the fatty acid derivatives that are shorter than normal intracellular fatty acid metabolites, and introduced a novel synthetic pathway for the biosynthesis of short-chain alkanes. This allowed the development of platform E. coli strain capable of producing gasoline for the first time. Furthermore, this platform strain, if desired, can be modified to produce other products such as short-chain fatty esters and short-chain fatty alcohols.
In this paper, the Korean researchers described detailed strategies for 1) screening of enzymes associated with the production of fatty acids, 2) engineering of enzymes and fatty acid biosynthetic pathways to concentrate carbon flux towards the short-chain fatty acid production, and 3) converting short-chain fatty acids to their corresponding alkanes (gasoline) by introducing a novel synthetic pathway and optimization of culture conditions. Furthermore, the research team showed the possibility of producing fatty esters and alcohols by introducing responsible enzymes into the same platform strain.
Professor Sang Yup Lee said, "It is only the beginning of the work towards sustainable production of gasoline. The titer is rather low due to the low metabolic flux towards the formation of short-chain fatty acids and their derivatives. We are currently working on increasing the titer, yield and productivity of bio-gasoline. Nonetheless, we are pleased to report, for the first time, the production of gasoline through the metabolic engineering of E. coli, which we hope will serve as a basis for the metabolic engineering of microorganisms to produce fuels and chemicals from renewable resources."
This research was supported by the Advanced Biomass Research and Development Center of Korea (ABC-2010-0029799) through the Global Frontier Research Program of the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF), Republic of Korea. Systems metabolic engineering work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (NRF-2012-C1AAA001-2012M1A2A2026556) by MSIP through NRF.
Short-Chain Alkanes Generated from Renewable Biomass
This diagram shows the metabolic engineering of Escherichia coli for the production of short-chain alkanes (gasoline) from renewable biomass.
Nature Cover Page (September 29th, 2013)
2013.11.04 View 10915 -
A powerful strategy for developing microbial cell factories by employing synthetic small RNAs
The current systems for the production of chemicals, fuels and materials heavily rely on the use of fossil resources. Due to the increasing concerns on climate change and other environmental problems, however, there has been much interest in developing biorefineries for the production of such chemicals, fuels and materials from renewable resources. For the biorefineries to be competitive with the traditional fossil resource-based refineries, development of high performance microorganisms is the most important as it will affect the overall economics of the process most significantly. Metabolic engineering, which can be defined as purposeful modification of cellular metabolic and regulatory networks with an aim to improve the production of a desired product, has been successfully employed to improve the performance of the cell. However, it is not trivial to engineer the cellular metabolism and regulatory circuits in the cell due to their high complexity.
In metabolic engineering, it is important to find the genes that need to be amplified and attenuated in order to increase the product formation rate while minimizing the production of undesirable byproducts. Gene knock-out experiments are often performed to delete those metabolic fluxes that will consequently result in the increase of the desired product formation. However, gene knock-out experiments require much effort and time to perform, and are difficult to do for a large number of genes. Furthermore, the gene knock-out experiments performed in one strain cannot be transferred to another organism and thus the whole experimental process has to be repeated. This is a big problem in developing a high performance microbial cell factory because it is required to find the best platform strain among many different strains. Therefore, researchers have been eager to develop a strategy that allows rapid identification of multiple genes to be attenuated in multiple strains at the same time.
A Korean research team led by Distinguished Professor Sang Yup Lee at the Department of Chemical and Biomolecular Engineering from the Korea Advanced Institute of Science and Technology (KAIST) reported the development of a strategy for efficiently developing microbial cell factories by employing synthetic small RNAs (sRNAs). They first reported the development of such system in Nature Biotechnology last February. This strategy of employing synthetic sRNAs in metabolic engineering has been receiving great interest worldwide as it allows easy, rapid, high-throughput, tunable, and un-doable knock-down of multiple genes in multiple strains at the same time. The research team published a paper online on August 8 as a cover page (September issue) in Nature Protocols, describing the detailed strategy and protocol to employ synthetic sRNAs for metabolic engineering.
In this paper, researchers described the detailed step-by-step protocol for synthetic sRNA-based gene expression control, including the sRNA design principles. Tailor-made synthetic sRNAs can be easily manipulated by using conventional gene cloning method. The use of synthetic sRNAs for gene expression regulation provides several advantages such as portability, conditionality, and tunability in high-throughput experiments. Plasmid-based synthetic sRNA expression system does not leave any scar on the chromosome, and can be easily transferred to many other host strains to be examined. Thus, the construction of libraries and examination of different host strains are much easier than the conventional hard-coded gene manipulation systems. Also, the expression of genes can be conditionally repressed by controlling the production of synthetic sRNAs. Synthetic sRNAs possessing different repression efficiencies make it possible to finely tune the gene expression levels as well. Furthermore, synthetic sRNAs allow knock-down of the expression of essential genes, which was not possible by conventional gene knock-out experiments.
Synthetic sRNAs can be utilized for diverse experiments where gene expression regulation is needed. One of promising applications is high-throughput screening of the target genes to be manipulated and multiple strains simultaneously to enhance the production of chemicals and materials of interest. Such simultaneous optimization of gene targets and strains has been one of the big challenges in metabolic engineering. Another application is to fine tune the expression of the screened genes for flux optimization, which would enhance chemical production further by balancing the flux between biomass formation and target chemical production. Synthetic sRNAs can also be applied to finely regulating genetic interactions in a circuit or network, which is essential in synthetic biology. Once a sRNA scaffold-harboring plasmid is constructed, tailor-made, synthetic sRNAs can be made within 3-4 days, followed by the desired application experiments.
Dr. Eytan Zlotorynski, an editor at Nature Protocols, said "This paper describes the detailed protocol for the design and applications of synthetic sRNA. The method, which has many advantages, is likely to become common practice, and prove useful for metabolic engineering and synthetic biology studies."
This paper published in Nature Protocols will be useful for all researchers in academia and industry who are interested in the use of synthetic sRNAs for fundamental and applied biological and biotechnological studies.
This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (NRF-2012-C1AAA001-2012M1A2A2026556) and the Intelligent Synthetic Biology Center through the Global Frontier Project (2011-0031963) of the Ministry of Science, ICT and Future Planning through the National Research Foundation of Korea.
2013.10.31 View 8794
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High Efficiency Bio-butanol production technology developed
KAIST and Korean Company cooperative research team has developed the technology that increases the productivity of bio-butanol to equal that of bio-ethanol and decreases the cost of production.
Professor Lee Sang Yeop (Department of Biological-Chemical Engineering) collaborated with GS Caltex and BioFuelChem Ltd. to develop a bio-butanol production process using the system metabolism engineering method that increased the productivity and decreased the production cost.
Bio-butanol is being widely regarded as the environmentally friendly next generation energy source that surpasses bio-ethanol.
The energy density of bio-butanol is 29.9MJ (mega Joule) per Liter, 48% larger than bio-ethanol (19.6MJ) and comparable to gasoline (32MJ). Bio-butanol is advantageous in that it can be processed from inedible biomass and is therefore unrelated to food crises.
Especially because bio-butanol shows similar characteristics especially in its octane rating, enthalpy of vaporization, and air-fuel ratio, it can be used in a gasoline engine.
However barriers such as difficulty in gene manipulation of producer bacterium and insufficient information prevented the mass production of bio-butanol.
Professor Lee’s team applied the system metabolism engineering method that he had invented to shift the focus to the production pathway of bio-butanol and made a new metabolism model.
In the new model the bio-butanol production pathway is divided into the hot channel and the cold channel.
The research team focused on improving the efficiency of the hot channel and succeeded in improving the product yield of 49% (compared to theoretical yield) to 87%.
The team furthered their research and developed a live bio-butanol collection and removal system with GS Caltex. The collaboration succeeded in producing 585g of butanol using 1.8kg of glucose at a rate of 1.3g per hour, boasting world’s highest concentration, productivity, and rate and improving productivity of fermentation by three fold and decreasing costs by 30%.
The result of the research was published in world renowned ‘mBio’ microbiology journal.
2012.12.21 View 8534
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Professor Moon Song Chun appointed representative director of European IT society
Professor Moon Song Chun from the College of business at KAIST was appointed as the representative director of Asia for the European IR society EUROMICRO at its 35th general meeting in Lille, France.
Professor Moon is highly regarded in his work in popularization of IT in 3rd world countries and has published the largest number of papers in the history of EUROMICRO. For the next two years, Professor Moon will work to introduce Asia’s IT capabilities to the world and to increase the recognition of the society in the region.
Professor Moon, who is also known as the first Computer Science Doctor (PhD) in Korea, has worked to popularize IT by initiating IT volunteer services in Africa, the Middle East, Central and South America, South East Asia and Eastern Europe. He has also helped in the recognition of Korea’s IT capacity, working as a Korean Delegate for the UN International Y2K Cooperation Center, a Distinguished Scholar at Hungarian Academy of Sciences, and a Visiting Scholar at Cambridge University.
2012.09.25 View 8181
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Liver Damage Mechanism of Hepatitis C Proven
KAIST researchers found mechanics behind a Hepatitis C virus, thereby taking a step closer to the development of a cure for Hepatitis C.
Professor Choi Chul Hui (Department of Biological and Brain Engineering) and Professor Shin Eui Chul (Graduate School of Medical Sciences) proved, for the first time in the world, the mechanism behind liver damage of a patient with Hepatitis C.
It is anticipated that this discovery will allow for the development of a Hepatitis C cure that has no side effects and little Liver damage.
Hepatitis C is an immune response of the body to the Hepatitis C virus and causes liver irritation.
Around 170million people are infected with Hepatitis C worldwide including 1% of the Korean population. Once infected, most cases turn into chronic cases and may lead to liver cancer.
However it was impossible to infect Hepatitis C within a test tube cell environment until 2005 and up till then Chimpanzees were used to study the virus which proved to be a huge barrier to research.
The research team used cells infected with Hepatitis C virus and found out that the virus works by increasing the destruction of cells by the TNF-a protein responsible for the cell’s immune response.
In addition the protein structure of the virus that causes this reaction was successfully found.
Conventionally the Hepatitis C medication focused on the suppressing the growth of the virus and therefore had many side effects.
The experimental results allow new medication aimed at suppressing the actual mechanism of liver damage to be discovered.
The result was selected as the cover dissertation of the September Edition of the Hepatolog magazine.
2012.09.11 View 11461
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Professor Kyung Wook Baek Wins the Best Thesis Award at the 2012 Pan-Pacific Microelectronic Symposium
Prof. Kyung Wook Baek from KAIST"s material science department has won the Best Thesis Award at the 2012 Pan-Pacific Microelectronic Symposium.
The title of this thesis was "Recent Advances in Anisotropic Conductive Adhesives Technology : Materials and Processes". Prof Baek had the honor of having his thesis be appointed the best thesis of the symposium.
This thesis includes his 15 years of research on ACAs which are a key element of display and semiconductor packaging technology.
Prof. Baek"s research results has been recognized as incredibly innovative in the field of ACAs and ultrasonic connection devices. This thesis has been recognized as setting the foundation for commercialization by professionals from all over the world at the symposium.
Prof. Baek has announced two innovative technologies on ACAs at the symposium.
One is a technology that merges the nanofiber technology with the ATAs. This technology was highly applauded for overcoming the problem of electric connection in micro-pitch display semiconductors, and successfully applying this to electronic packaging materials.
Currently, commercialization process based on the patent is ongoing. It is expected that we will be able to take hold of the entire market once the commercialization succeeds.
The other technology was to improve the liability and overcome the limits of the current flow in ACAs through the use of solder molecules. This is also undergoing commercialization process for use in mobile electronic devices.
Together with this, Prof.Baek has reported an innovative case where the original heat compression process was replaced with a new ultrasonic process. This discovery is deemed to be extremely great due to its implications in replacing all heat compression systems. This too will soon be commercialized
Prof.Baek has played a crucial role in the development of electronic packaging material and processing technology. He has written the largest number of theses in this area, and has proven himself to be the world"s best through winning this award.
2012.05.10 View 9420
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Professor Kang Suk Joong receives 'Korea Engineering Award.'
KAIST”s Professor Kang Suk Joong of the Department of Material Science and Engineering received ‘Korea Engineering Award’ from the Ministry of Education, Science and Technology and Korea Research Foundation. The award is given to those professors who have accomplished world class research and results.
Professor Kang has potentially redirected the direction of research in the field of the microstructure of materials by explaining the fundamental principle behind how the microstructure of a material that affects the physical properties of the polycrystalline structure and changes through processing. Professor Kang applied the results of his findings in the manufacture of new materials and made significant contributions to Korean Material Engineering Industry and was consequently awarded the award.
The ‘Korea Engineering Award’ was thought of in 1994 and a total of 24 recipients were recognized through the award in various fields like electronics, mechanics, chemistry, construction, etc. The recipient is awarded in addition the President’s award and 50million won as prize money.
The ceremony for ‘Korea Engineering Award’ and the ‘Young Scientist Award’ was held in Seoul Press Center Press Club on the 22nd of December at 3pm. The Minister of Education, Science and Technology (Lee Joo Ho), member of Board of Directors of the Korea Research Foundation (Kim Byoung Gook), Director of Korea Science and Technology Archive (Jeong Gil Seng), along with the recipients attended the ceremony.
In addition, Professor Kang was appointed as Distinguished Professor in March 2010 in recognition of his research accomplishments.
2011.01.18 View 10522